KU-55933 [2-morpholino-6-(thianthren-1-yl)-4H-pyran-4-one] is a
novel, specific and very potent small molecule inhibitor of Ataxia-Telangiectasia Mutated (ATM) kinase. The
serine/threonine protein kinase ATM signals to cell cycle and DNA repair
components by phosphorylating downstream targets such as p53, CHK2, NBS1, and
BRCA1. Mutation of ATM occurs in the human autosomal recessive disorder
ataxia-telangiectasia, which is characterized by hypersensitivity to ionizing
radiation and a failure of cells to arrest the cell cycle after the induction
of DNA double-strand breaks. It has thus been proposed that ATM inhibition
would cause cellular radio- and chemosensitization [1].
The activity
of KU-55933 is as follows:
IC50 (ATM enzyme assay) = 12.9 ± 0.1 nM
IC50 (ATM enzyme assay) = 12.9 ± 0.1 nM
Ki (ATP) = 2.2 ± 0.3 nM
Common Name: KU-55933
Synonyms: KU 55933; KU-55933; KU55933
IUPAC Name: 2-morpholino-6-(thianthren-1-yl)-4H-pyran-4-one
CAS Number: 587871-26-9
Mechanism of Action: Kinase Inhibitor; ATM Inhibitor
Indication: Various Cancers
Development Stage: Investigational
Company: KuDOS Pharmaceuticals
The cellular
response to DNA damage is one of coordinated repair and moderation of
proliferation. ATM is a protein kinase member of the phosphatidylinositol
3'-kinase (PI3K)–related kinase (PIKK) family that plays a critical role in the
maintenance of genome integrity . The Mr 345 000 phospho-protein plays key
roles in coordinating cellular responses to IR-induced DNA double-strand
breaks. Mutation of ATM occurs in the human autosomal recessive disorder
ataxia-telangiectasia (A-T), which is characterized by several symptoms,
including cerebellar degeneration, oculocutaneous telangiectasia, growth
retardation, immune deficiencies, and characteristics of premature aging. At
the cellular level, A-T is characterized by a hypersensitivity to ionizing
radiation and radiomimetic drugs, radioresistant DNA synthesis, and chromosomal
instability. In addition, there is a failure of A-T cells to arrest the cell
cycle after DNA damage, thus reducing the opportunity for the repair of the
genome before DNA replication or mitosis. Exposure of normal cells to ionizing
radiation results in cell cycle arrest and ATM appears to be critical for the
induction of these events, orchestrating the response by the downstream
signaling to other response factors [1].
KU-55933 exhibits
an ATP competitive mechanism of action. Importantly, this potency is
essentially restricted to ATM as it is at least 100-fold more potent against
ATM than against the other PIKK family members tested and shows no activity
against a panel of 60 unrelated kinases. Of key importance here was that the
closest family member with regards to homology across the ATP-binding domain,
ATR, was not inhibited at doses up to 100 µmol/L. Moreover, a classic hallmark
of cells derived from A-T patients is their extreme sensitivity to ionizing
radiation. KU-55933 can recapitulate this phenotype in a range of cell lines
and that it does not additionally sensitize A-T cells to the effects of
ionizing radiation [1].
KU-55933
shows specificity with respect to inhibition of other phosphatidylinositol
3'-kinase-like kinases. Cellular inhibition of ATM by KU-55933 was demonstrated
by the ablation of ionizing radiation-dependent phosphorylation of a range of
ATM targets, including p53, yH2AX, NBS1, and SMC1. KU-55933 did not show
inhibition of UV light DNA damage induced cellular phosphorylation events.
Exposure of cells to KU-55933 resulted in a significant sensitization to the
cytotoxic effects of ionizing radiation and to the DNA double-strand
break-inducing chemotherapeutic agents, etoposide, doxorubicin, and
camptothecin. Inhibition of ATM by KU-55933 also caused a loss of ionizing
radiation-induced cell cycle arrest. By contrast, KU-55933 did not potentiate
the cytotoxic effects of ionizing radiation on ataxia-telangiectasia cells, nor
did it affect their cell cycle profile after DNA damage.
ATM,
a protein deficient in patients with ataxia-telangiectasia disease, is
traditionally considered as a nuclear protein kinase that functions as a signal
transducer in response to DNA damage. It has recently been shown that ATM is
also a cytoplasmic protein that mediates the full activation of Akt in response
to insulin. Aberrant activation of Akt plays a pivotal role in cancer
development. KU-55933, blocks the phosphorylation of Akt induced by insulin and
insulin-like growth factor I in cancer cells that exhibit abnormal Akt
activity. Moreover, KU-55933 inhibits cancer cell proliferation by inducing G1
cell cycle arrest. It does so through the downregulation of the synthesis of
cyclin D1, a protein known to be elevated in a variety of tumors. In addition,
KU-55933 treatment during serum starvation triggers apoptosis in these cancer
cells. Results suggest that KU-55933 may be a novel chemotherapeutic agent
targeting cancer resistant to traditional chemotherapy or immunotherapy due to
aberrant activation of Akt. Furthermore, KU-55933 completely abrogates
rapamycin-induced feedback activation of Akt. Combination of KU-55933 and
rapamycin not only induces apoptosis, which is not seen in cancer cells treated
only with rapamycin, but also shows better efficacy in inhibiting cancer cell
proliferation than each drug alone. Therefore, combining KU-55933 with
rapamycin may provide a highly effective approach for improving mammalian
target of rapamycin–targeted anticancer therapy that is currently hindered by
rapamycin-induced feedback activation of Akt [3].
References:
1. Hickson, I.; et. al.
Identification and Characterization of a Novel and Specific Inhibitor of the
Ataxia-Telangiectasia Mutated Kinase ATM. Cancer Res 2004, 64(24), 9152-9159.
2. Cockcroft, X.-L. F.; et.
al. Pyranones useful as atm inhibitors. WO2003070726A1
3. Li, Y.; et. al. The ATM inhibitor KU-55933
suppresses cell proliferation and induces apoptosis by blocking Akt in cancer
cells with overactivated Akt. Mol Cancer Ther 2010, 9(1), 113-125.